mouse monoclonal anti cd21 antibody  (Novus Biologicals)

Journal: Bioengineering & Translational Medicine

Article Title: Microbead‐based synthetic niches for in vitro expansion and differentiation of human naïve B‐cells

doi: 10.1002/btm2.10751

Figure Lengend Snippet: FIGURE 5 Synergistic activation by MB-CD40L, BCR, and TLR-9 signaling for improved B-cell expansion and differentiation. (a) Representative flow cytometry plot of CD27 versus CD38 for days 0–13 cells (gated on IgD- B-cells from day-4 onwards) for the activation of naïve B-cells by MB-CD40L with or without the B-cell receptor signal by anti-human IgM/G/A (H + L) antibody plus co-receptor signal by anti- human CD21 antibody (referred to as BCR) and/or toll-like receptor signal by the TLR-9 agonist CpG ODN 2006 in the presence of BAFF and the interleukins Il-2, -4, -10 and -21. Bar graphs of (b) fold expansion and the yield of (c) memory B-cells and (d) ASCs on day-13 for each group. Data were acquired each day as duplicates for each group and represented as mean + standard deviation. Statistical analysis was conducted by applying ordinary one-way ANOVA with Tukey's multiple comparisons (*p < 0.05; **p ≤0.01; ***p ≤0.001; ns p > 0.05).

Article Snippet: For the activation of the B-cell receptor, a combination of goat anti-human IgA + IgG + IgM (H + L) antibody (109006064; Jackson Immuno Research Laboratories, USA) was added to the culture at 6 μg/mL concentration, together with a monoclonal mouse anti-human CD21 antibody (MAB4909; R&D Systems, USA) at 1 μg/mL.

Techniques: Activation Assay, Flow Cytometry, Standard Deviation

Journal: Veterinary Research

Article Title: Baicalin attenuates PD-1/PD-L1 axis-induced immunosuppression in piglets challenged with Glaesserella parasuis by inhibiting the PI3K/Akt/mTOR and RAS/MEK/ERK signalling pathways

doi: 10.1186/s13567-024-01355-1

Figure Lengend Snippet: The effects of baicalin, levamisole, and BMS-1 on the proportions of CD3 + CD8 + T cells among splenocytes and blood and the proportion of CD3 – CD21 + B cells among splenocytes were measured by flow cytometry. Blood was collected, the spleen was collected, and splenocytes were prepared. The proportions of CD3 + CD8 + T cells and CD3 – CD21 + B cells were determined by flow cytometry. A Proportion of CD3 + CD8 + T cells among splenocytes; B proportion of CD3 + CD8 + T cells among blood cells after 24 h; C proportion of CD3 + CD8 + T cells among blood cells after 48 h; D proportion of CD3 – CD21 + B cells among splenocytes; BA: Baicalin; ## P < 0.01 versus controls; ### P < 0.001 versus controls; *significance at P < 0.05; **significance at P < 0.01; ***significance at P < 0.001.

Article Snippet: Following staining with mouse anti-porcine CD3ε-FITC (Cat. No. 4510-02, Monoclonal), mouse anti-porcine CD4-SPRD (Cat. No. 4515-13, Monoclonal), mouse anti-porcine CD8a-PE (Cat. No. 4520-09, Monoclonal) and mouse anti-porcine CD21-PE (Cat. No. 4530-09, Monoclonal) (all from SouthernBiotech, Birmingham, AL, USA) to gate for CD3 T cells, CD4 T cells, CD8 T cells and CD21 B cells, the cells were stained with CD3ε-FITC-CD4-SPRD, CD3ε-FITC-CD8a-PE or CD3ε-FITC-CD21-PE to generate a four-quadrant gate.

Techniques: Flow Cytometry